Part:BBa_K1391007:Experience

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Applications of BBa_K1391007

This is fusion protein comprising Syk and tobacco etch virus (TEV) protease, joined by a glycine-serine linker.

Spleen Tyrosine Kinase (Syk) is a tyrosine kinase that has a high affinity for the phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) of the CD79A and CD79B proteins (components of the B-Cell Receptor Complex). Syk binds to the phosphorylated ITAM and the ITAM then phosphorylates Syk allowing Syk to initiate a downstream signalling cascade that ordinarily results in the proliferation of B-Cells during clonal selection.

TEV protease is a protease that cleaves at a particular amino acid sequence. In our synthetic B-cell receptor system, a transcriptional activator (Gal4VP16) is fused using a TEV protease cleavage site to the intracellular tails of CD79A and/or CD79B. When the Syk-TEVp fusion is recruited to our engineered receptor upon antigen binding, the TEV protease cleaves at its cleavage site on the CD79A and/or CD79B fusions and releases a transcriptional activator.

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